This course applies the concepts of reaction rate, stoichiometry and equilibrium to …
This course applies the concepts of reaction rate, stoichiometry and equilibrium to the analysis of chemical and biological reacting systems, derivation of rate expressions from reaction mechanisms and equilibrium or steady state assumptions, design of chemical and biochemical reactors via synthesis of chemical kinetics, transport phenomena, and mass and energy balances. Topics covered include: chemical/biochemical pathways; enzymatic, pathway, and cell growth kinetics; batch, plug flow and well-stirred reactors for chemical reactions and cultivations of microorganisms and mammalian cells; heterogeneous and enzymatic catalysis; heat and mass transport in reactors, including diffusion to and within catalyst particles and cells or immobilized enzymes.
Genetic mutations provide valuable tools for analyzing biochemical pathways in yeast. In this …
Genetic mutations provide valuable tools for analyzing biochemical pathways in yeast. In this module, students use deletion mutants to analyze the steps in methionine biosynthesis in Saccharomyces cerevisiae. At the end of this module, students will be able to:understand and use the correct genetic nomenclature for genes, proteins and mutant strainsexplain how genetic screens are used to isolate mutant strains with particular phenotypesdistinguish various met deletion strains by their ability to grow on selective media containing different sulfur sources and by their appearnace on indicator mediapredict how mutations in various MET genes will affect the intracellular concentrations of intermediates in the methionine biosynthesis This module is part of a semester-long introductory lab class, Investigations in Molecular Cell BIology, at Boston College.
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