Western blots are widely used in molecular cell biology to identify proteins-of-interest in complex protein samples. In western blots, antibodies and detection reagents are used to stain membranes containing replicas of polyacrylamide gels. This module introduces students to the theory and practice of western blots. In this module, students:learn about the biological origins and structural properties of antibodies that underly their specificity.learn how epitope tags allow the detection of proteins-of-interest on western blots.prepare membrane replicas containing protein extracts that have been resolved by SDS-PAGE.analyze protein expression in yeast that have been transformed with expression plasmids.This module is part of an introductory laboratory course, Investigations in Molecular Cell Biology, at Boston College.
- Subject:
- Biology
- Chemistry
- Genetics
- Health, Medicine and Nursing
- Material Type:
- Module
- Author:
- Clare OConnor
- Date Added:
- 09/05/2018