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Spectrophotometry, Spectroscopy, and Protein Determinations
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We are all well aware of the composition of the world -atoms form molecules, compound become more complex, and the organization of these atoms into materials with unique structures is what brings about life. As scientists though, we must study these substances , which presents a challenge. How do we study something so incredibly small? One of the simplest methods is spectrophotometry. Different molecules will interact with light in different ways. By studying this, we can quantitatively say both how much light a compound absorbs as well as what kind of light. Certain functional groups tend to absorb light at certain wavelengths, giving "peaks" to the spectrum of light absorption. This lab demonstrates basic principles of absorbance, measured using spectrophotometers.

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