Author:
Clare OConnor
Subject:
Biology, Chemistry
Material Type:
Module
Level:
Community College / Lower Division, College / Upper Division, Career / Technical
Tags:
  • Agarose
  • Agarose Gel
  • Gel Electrophoresis
    License:
    Creative Commons Attribution Non-Commercial Share Alike
    Language:
    English
    Media Formats:
    Downloadable docs, Video

    Agarose gel electrophoresis

    Agarose gel electrophoresis

    Overview

    In this module, students prepare and run an agarose gel that they use to separate DNA molecules of various sizes. Students stain the gels with ethidium bromide to visualize the positions of DNA molecules. Students estimate the sizes of separated DNA molecules by their migration distances relative to those of molecular weight standards. 

    This module is part of a semester-long introductory lab class, Investigations in Molecular Cell Biology, at Boston College. 

    Section 1

    In this module, students prepare and run an agarose gel that they use to separate DNA molecules of various sizes. Students stain the gels with ethidium bromide to visualize the positions of DNA molecules. Students estimate the sizes of separated DNA molecules by their migration distances relative to those of molecular weight standards. 

    This module is part of a semester-long introductory lab class, Investigations in Molecular Cell Biology, at Boston College.