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Overview:
This module introduces students to the theory and practice of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE provides a simple method for separating proteins in complex mixtures by their molecular weights. At the end of this module, students will:understand the principles that govern protein separation on discontinuous SDS- PAGE gels.be able to cast and run SDS-PAGE gels.be able to analyze the pattern of bands in yeast cell extracts on a stained SDS-PAGE gel.be able to calculate the molecule weight of a protein from its migration on SDS-PAGE gels.This module is part of a semester-long introductory laboratory course, Investigations in Molecular Cell Biology, at Boston College.
Subject:
Biology, Genetics, Chemistry
Level:
Community College / Lower Division, College / Upper Division, Career / Technical
Material Type:
Module
Author:
Date Added:
09/05/2018
License:
Creative Commons Attribution Non-Commercial Share Alike Creative Commons Attribution Non-Commercial Share Alike
Language:
English
Media Format:
Downloadable docs, Video

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