Leigh-Ann Cruz IUPUI NEST PPT.pptx
Nanomedicine: Gas Biosensors for Disease Diagnostics
Overview
Nanotechnology is the study of technology at the nanoscale(1-100 nm) used to create functional materials, devices, and systems through understanding and control of matter at these dimensions. Nanomedicine is now being used to treat and diagnose diseases; we will be constructing gas biosensors to test for the change in resistance to the presence of acetone and isopropyl alcohol.
Standards
NGSS Standards:
HS-ETS1-1.Analyze a major global challenge to specifyqualitative and quantitative criteria and constraintsfor solutions thataccount for societal needs and wants.
HS-ETS1-2.Design a solution to a complex real-worldproblem by breaking it down into smaller, more manageableproblems thatcan be solved through engineering.
HS-PS2-6.Communicate scientific and technical informationabout why the molecular-level structure is importantin thefunctioning of designed materials.
HS-PS3-5.Develop and use a model of two objects interactingthrough electric or magnetic fields to illustratethe forces betweenobjects and the changes in energy of the objects dueto the interaction.
IDOE Science Standards:
SEPS.4 Analyzing and interpreting data
SEPS.5 Using mathematics and computational thinking
Learning Objectives
- Describe ways nanotechnology is expected to influencesociety.
- List key areas of research in the nanotechnology fieldand real-world applications.
- Explain the length scale of nanotechnology relativeto traditional length scales.
- Convert measurements to different units.
- Explain how nanoscience works as it pertains to real-worldapplications.
- Identify key applications and potential applicationsfor specific nanotechnologies.
Background
Teacher Information/Concepts:
Nanotechnology is the study of technology at the nanoscale(1-100 nm) used to create functional materials, devices, and systems through understanding and control of matter at these dimensions. Nanomedicine is now being used to treat and diagnose diseases; we will be constructing gas biosensors to test for the change in resistance to the presence of acetone and isopropyl alcohol.
Prior Student Knowledge and Possible Preconceptions/Misconceptions:
- The nanoscale is small (but exactly *how* small *is* nano?)
- Electricity is the movement of electrons (how exactly do the electrons move?)
Materials
- Scour Pads
- Multimeters
- Alligator Test Clips and Leads
- Copper Etchant Solution (Ferric Chloride)
- Gloss Photo Paper
- Crystallizing Dish
- Acetone
- Laminating Machine
- Tweezers
- Hot Plate
Procedure
See lesson plan and resources attached in the materials section.
Vocabulary:
- nano
- nanometer
- nanotechnology
- photolithography
- biosensor
- electricity
- voltage
- current
- resistance
Adapted from the IDOE
Hook/Set the Stage-Nano Nature Quiz: Students will guess at what objects are photographed at the nano scale-What is Nano? Video
Procedure: Day 1
- Intro to Nanomedicine and Biosensors
15 min - [Warm-Up]a) Edmodo Self-Quiz: Nano Nature ID Quizb)
What is Nano? Video (https://www.youtube.com/watch?v=Dxq-ffp3jqA)
15 min - [Intro to Nano Discussion]-Size of Nano-History of Nano (Gold used in stained glass, buckyball,etc.)-Definition of Nanotechnology-Properties of atoms/chemical rxns at nano-scale-Nanomaterials-Nanoapplications → Spiral into Nanomedicine
10 min - [Nanomedicine Discussion]-Guiding Questions:-Why is nanotechnology important in the realm of medicine?-What are examples of nanotechnology in medicine?-What are examples of how a specific disease can bemonitored or detected?-Spiral into Biosensors (act like dog noses that sniffout disease)
10 min - [Dogs Diagnose Disease Videos]-Ted-Ed: How Dogs Smell Video (https://www.youtube.com/watch?v=p7fXa2Occ_U)-Ovarian Cancer Sniffing Dogs(https://www.webmd.com/cancer/cutting-edge-16/video-early-cancer-detection-robin-roberts)
20 min - [Electricity Refresher + Intro to PhotolithographyNotes]-Voltage, Current, and Resistance Diagram
-Helpful Videos-What is Electricity?https://www.youtube.com/watch?time_continue=1&v=oB1v-wh7EGU
-Voltage, Current, and Resistance Basicshttps://www.youtube.com/watch?v=m75jvmtVyVY
-Voltage, Current, and Resistancehttps://www.youtube.com/watch?v=nBi662ZRfTA
-High surface to volume ratio of nanotechnology applies to intercalating sensor design and interactions of carbon nanotubes
-Photolithography Videohttps://www.youtube.com/watch?v=oBKhN4n-EGI-We will use thermolithography (using thermal heat to transfer a Mask onto the copper substrate) to create a Copper-clad Biosensor Chip to detect acetone (found in the breath of type 1 diabetics) and isopropyl alcohol (found in the breath of those with COPD and liver disease)
15 min - [Biosensor Chip Design] Design BiosensorChip and explain why you think this design will be effective
Homework - Take Pre-Lab Self-Quiz on Edmodo
Day 2 - Copper Thermolithography + Gas Biosensor Lab
10 min - [Warm-Up] Pre-Lab Questions
45 min - [Thermolithography]-Transfer Mask-Etching (~30 min)
30 min - [Gas Biosensor Testing and Post-Lab Questions]-Measure percent change in resistance of acetone and isopropyl alcohol compared to control (ambient air)
Extra Down Time/Homework
- [Intro to InformercialPresentation]
-Create a novel (feasible) biosensor and film an infomercial to inform, educate, and sell your product
Credits
This learning module was created by Leigh-Ann Cruz, a participant in Indiana University-Purdue University’s NSF-Funded “Nanotechnology Experiences for Students and Teachers (NEST)” Program (Award # 1513112).