Micrograph Escherichia coli and Mycobacterium smegmatis acid fast 1000X p000069
This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Escherichia coli cells and Mycobacterium smegmatis cells grown separately in broth culture at 37 degrees Celsius. The cells were heat-fixed to a slide and acid-fast stained using the Ziehl-Neelsen method prior to visualization. M. smegmatis (acid-fast) stains pink with carbol fuchsin. E.coli (non-acid-fast) stains blue with methylene blue counterstain.
Image credit: Emily Fox
Light background with many blue rods and clumped, pink rods.