Author:
Emily Fox
Subject:
Environmental Science, Health, Medicine and Nursing, Biology
Material Type:
Diagram/Illustration
Level:
Community College / Lower Division, College / Upper Division
Tags:
  • Bacteria
  • Microbiology
  • Micrograph
  • Microscope
  • Microscopy
  • Stain
  • License:
    Creative Commons Attribution
    Language:
    English
    Media Formats:
    Graphics/Photos

    Micrograph Escherichia coli and Mycobacterium smegmatis acid fast 1000X p000069

    Micrograph Escherichia coli and Mycobacterium smegmatis acid fast 1000X p000069

    Overview

    This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Escherichia coli cells and Mycobacterium smegmatis cells grown separately in broth culture at 37 degrees Celsius. The cells were heat-fixed to a slide and acid-fast stained using the Ziehl-Neelsen method prior to visualization. M. smegmatis (acid-fast) stains pink with carbol fuchsin. E.coli (non-acid-fast) stains blue with methylene blue counterstain.

    Image credit: Emily Fox

    Micrograph

    Light background with many blue rods and clumped, pink rods.Light background with many blue rods and clumped, pink rods.