The text introduces students to molecular cell biology within the context of a semester-long research project in functional genomics. In the Pathways over Time project, students study the evolutionary conservation of genes in methionine synthesis. Each chapter includes both theoretical background material as well as detailed experimental procedures. Chapters can be used alone or in combination, depending on the course.

The pages represent an online biology textbook created by Professor John W. Kimball who taught at Harvard, Tufts, and Phillips Andover Academy before he retired. He now maintains this free biology book, constantly updated and set up for easy browsing and searching.

This laboratory manual was developed for a microbiology laboratory course designed to give students in the health care professions basic knowledge and skills of the techniques used to study microbes. The course is taken by first-time college students in the 6-year medical program and by prepharmacy undergraduates. Because this is the only microbiology laboratory course these students take, the laboratory experiments are essential to illustrate microbiological principles and methods presented in lecture companion course. The laboratory exercises demonstrate basic concepts of microbiology with emphasis on infectious diseases and host defenses.

Throughout the course, students gain competency in the following areas:

Safe handling of microbes
Knowledge of the techniques and media used to subculture microbes
Use of the light microscope
Staining techniques
Quantitative methods
Identification of microbes using biochemical tests and/or immunological techniques
Interpretation of experimental results

In the past, I supplemented a commercially published lab manual with detailed weekly instructions posted to the course website. My instructions summarized the theory presented, pointing out the important concepts. Based on past experience, I made changes to the lab procedure accommodating organisms that work well in the UMKC teaching lab. In addition, the instructions gave students clarification on the post lab questions, encouraging critical thinking and evaluation of their actual experimental results. Students were required to use both the manual and my handouts to fully understand the exercise. As much as I tried to make each week’s activities clear, there was often confusion about the procedure, observations and/or expectations on the post-lab questions. This work aims to put it all together in one place for the student. For this project, I have built upon much of my original supplementary material using several open educational resources, most notably, OpenStax Microbiology. I appreciate the funding and support from the UM-system and the UMKC Libraries. I am grateful to my students who make teaching fun and interesting and will be unwitting editors and evaluators of this work. Sincerely,

Loretta Sanderson Klamm

This well-organized lab manual is housed in LibreTexts and each of the 21 labs begins by listing goals and student learning outcomes. Each lab contains useful images and highlights important equations and examples. The laboratory topics include basic lab skills and topics such as safety, metrics & measurements, pipetting, microscopy, spectrophotometry, making buffers and measuring pH, DNA and protein gel electrophoresis, PCR, ELISA, performing restriction enzyme digests and transformation, and Good Manufacturing Practices (GMP). This lab manual provides specific directions as to how students should organize their corresponding laboratory notebook and each lab asks students specific conclusion questions that they are supposed to answer in their lab notebook.

The protocols and instructor guide included in this lab manual are part of a semester-long Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions. Students are tasked with designing and then engineering a biological device in a bacterium (E. coli) using standardized DNA parts called Bio-Bricks from the iGEM (www.iGEM.org) registry. The process of engineering the device requires iteration of standard molecular biology cloning techniques which are outlined in the lab manual, some including video protocols. Additionally, provided are recipes for reagents, a supply/resources list, and instructor recommendations. A series of faculty training videos were created to further help with the implementation of the SynBio CURE.

Human Anatomy Laboratory Manual is a diagram-based lab manual for 1-semester Human Anatomy courses. Included are over 100 openly-licensed images that students will be able to label and learn from.

This content was created as a lab manual to accompany the fully online lab associated with Life Science in a Changing Climate, a non-majors biology course. It contains mainly original content with some figures and images taken from open-resources. Each figure is attributed with the source page in the figure description, in accordance to each respective license. The content covers background information, the setup to the online lab activity, and a climate connection.

Word Count: 13496

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

Word Count: 8522

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

Charles Darwin, Evolution and Tropical Australia

Word Count: 4842

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

This chapter discusses the biological and behavioral aspects of romantic love and social bonding. Affiliative behaviors such as hugging and cuddling have a biological application, and research has found increased levels of nanopeptides such as oxytocin and vasopressin during such behaviors. These nanopeptides play a significant role in social bonding, and may also be involved in the development of romantic feelings. The chapter also discusses attachment theory, which suggests that the formation of a secure attachment relationship with a primary caregiver during early childhood is important in building confidence in the availability of attachment figures. The role of vasopressin, oxytocin, and their receptors in love and pair-bonding has been extensively studied in prairie voles, which form monogamous pairs that stay together for a lifetime.

Short Description:
Biology textbook based on the OpenStax biology book.

Word Count: 94091

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

Short Description:
Biology textbook based on the OpenStax biology book.

Word Count: 93199

ISBN: 978-1-63635-004-2

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

This book was created for upper division microbiology students studying virology. It will describe the molecular biology and major diseases of virus families that cause significant disease in animals and humans. This book is by no means meant to be exhaustive. In fact, because virology can be so overwhelming, the author has tried to keep the book as simple as possible, while still giving the reader a solid understanding of the molecular mechanisms of viral replication and pathogenesis.

This Microbiology textbook, adapted from the Boundless version, is intended to provide health care professionals with a working knowledge of infectious disease. Much of the book is devoted to the structure/function relationships in pathogenic microorganisms and how these relationships are expressed in host-parasite interactions. The text also examines Microbial diseases of specific organ systems as well as host response mechanisms.

Microbiology is the study of microorganisms, which are defined as any microscopic organism that comprises either a single cell (unicellular), cell clusters or no cell at all (acellular). This includes eukaryotes, such as fungi and protists, and prokaryotes. Viruses and prions, though not strictly classed as living organisms, are also studied.

Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Short Description:
Return to milneopentextbooks.org to download PDF and other versions of this textNewParaAs a group of organisms that are too small to see and best known for being agents of disease and death, microbes are not always appreciated for the numerous supportive and positive contributions they make to the living world. Designed to support a course in microbiology, Microbiology: A Laboratory Experience permits a glimpse into both the good and the bad in the microscopic world. The laboratory experiences are designed to engage and support student interest in microbiology as a topic, field of study, and career.NewParaThis text provides a series of laboratory exercises compatible with a one-semester undergraduate microbiology or bacteriology course with a three- or four-hour lab period that meets once or twice a week. The design of the lab manual conforms to the American Society for Microbiology curriculum guidelines and takes a ground-up approach -- beginning with an introduction to biosafety and containment practices and how to work with biological hazards. From there the course moves to basic but essential microscopy skills, aseptic technique and culture methods, and builds to include more advanced lab techniques. The exercises incorporate a semester-long investigative laboratory project designed to promote the sense of discovery and encourage student engagement. The curriculum is rigorous but manageable for a single semester and incorporates best practices in biology education.

Long Description:
As a group of organisms that are too small to see and best known for being agents of disease and death, microbes are not always appreciated for the numerous supportive and positive contributions they make to the living world. Designed to support a course in microbiology, Microbiology: A Laboratory Experience permits a glimpse into both the good and the bad in the microscopic world. The laboratory experiences are designed to engage and support student interest in microbiology as a topic, field of study, and career.

Word Count: 37934

ISBN: 978-1-942341-54-3

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

Short Description:
This book is a derivation of the OpenStax Microbiology textbook and is written for microbiology majors, non-majors and allied health students.

Long Description:
This book is a derivation of the OpenStax Microbiology textbook and is written for microbiology majors, non-majors and allied health students. This first edition includes more basic microbiology, microbial physiology and microbial ecology, as well as more up-to-date information and Canadian examples.

Word Count: 456009

Included H5P activities: 296

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

This Canvas shell is created for biology instructors using the OpenStax Microbiology textbook. Each module contains quizzes and activities for one chapter of the textbook. Each quiz is designed to randomly pull 5 questions from a question bank. The activities are created in Canvas new quiz and H5P. Each chapter contains a case study based on the 'Clinical focus' of the chapter in the OpenStax textbook.