This well-organized lab manual is housed in LibreTexts and each of the 21 labs begins by listing goals and student learning outcomes. Each lab contains useful images and highlights important equations and examples. The laboratory topics include basic lab skills and topics such as safety, metrics & measurements, pipetting, microscopy, spectrophotometry, making buffers and measuring pH, DNA and protein gel electrophoresis, PCR, ELISA, performing restriction enzyme digests and transformation, and Good Manufacturing Practices (GMP). This lab manual provides specific directions as to how students should organize their corresponding laboratory notebook and each lab asks students specific conclusion questions that they are supposed to answer in their lab notebook.
The protocols and instructor guide included in this lab manual are part of a semester-long Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions. Students are tasked with designing and then engineering a biological device in a bacterium (E. coli) using standardized DNA parts called Bio-Bricks from the iGEM (www.iGEM.org) registry. The process of engineering the device requires iteration of standard molecular biology cloning techniques which are outlined in the lab manual, some including video protocols. Additionally, provided are recipes for reagents, a supply/resources list, and instructor recommendations. A series of faculty training videos were created to further help with the implementation of the SynBio CURE.