If the dialysis is the most know method used for buffer exchange, other methods less as desalting and tangential flow filtrations can be useful to speed up or scale up the buffer exchange process.
The use of high cell density media in recombinant protein expression with E.coli may allow to scale down the culture volumes and increase the throughput.
Guidelines to easyl make your own ethanol based hand rub sanitizer
Some tips to prepare, load and run your agarose gel to analize your PCR product or digested vector!
in the following video you can find a list of useful free on-line tools for DNA sequence analisys.
Some tips to select, prepare and run SDS-page for your protein sample?
Guidelines to perfom a PCR screening from E.coli colonies
In the video you can find some hints to design a primers for clone your GOI in a plasmid using restriction enzyme and ligase cloning approach
I ProteoCool is a an open source with simple but Cool Protocols for newbies in the field of molecular biology and protein chemistry.
Check the video to see how you can stain and destain your SDS-page in few minutes thanks to the use of a microwave owen.
in the video you can find a list of usefull tools for analize the protien sequences as
- protein alligment
- topoligy prediction
- secondary structure calculation
- signal peptide identification
- search of template for modelling in the PDB
and many others