Introduction to Biotechnology II (BIOL1415), provides learners with a practical exploration of a regulated biotechnology workplace. This course is a continuation of Introduction to Biotechnology I (BIOL 1414), and the cornerstone for the Biotechnology Level-One Certificate, as it provides students ample opportunity to master entry-level laboratory workforce skills. This course builds on knowledge in biotechnology, chemistry, & biology, and provides workforce training in areas of regulatory documentation, equipment validation, and teamwork. The goals of this course are to develop core laboratory skills needed in a bioscience lab job; critical thinking and multitasking, teamwork and accountability, accuracy in calculations and experimental analysis, and demonstrate skills associated with working in a regulated laboratory workplace.
The NBC2 Biomanufacturing Laboratory Manual is a companion piece to the Introduction to Biomanufacturing textbook. The lab manual is SOP-driven and follows the content order of the textbook. The lab manual guides students through hands-on laboratory activities, provides training on industry-grade equipment and introduces students to conducting experiments while following current Good Manufacturing Practices.
This WikiBooks textbook provides an outline of some of the major topics in biotechnology including the various fields of biotechnology, an introduction to the Code of Federal Regulations, laboratory notebooks and Standard Operating Procedures (SOP’S), upstream and downstream process development, and laboratory skills for a biotechnology technician. This textbook primarily consists of bulleted outlines rather than narrative text and would probably work better as an auxiliary resource for review rather than the main textbook assigned for a course.
This well-organized lab manual is housed in LibreTexts and each of the 21 labs begins by listing goals and student learning outcomes. Each lab contains useful images and highlights important equations and examples. The laboratory topics include basic lab skills and topics such as safety, metrics & measurements, pipetting, microscopy, spectrophotometry, making buffers and measuring pH, DNA and protein gel electrophoresis, PCR, ELISA, performing restriction enzyme digests and transformation, and Good Manufacturing Practices (GMP). This lab manual provides specific directions as to how students should organize their corresponding laboratory notebook and each lab asks students specific conclusion questions that they are supposed to answer in their lab notebook.
The protocols and instructor guide included in this lab manual are part of a semester-long Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions. Students are tasked with designing and then engineering a biological device in a bacterium (E. coli) using standardized DNA parts called Bio-Bricks from the iGEM (www.iGEM.org) registry. The process of engineering the device requires iteration of standard molecular biology cloning techniques which are outlined in the lab manual, some including video protocols. Additionally, provided are recipes for reagents, a supply/resources list, and instructor recommendations. A series of faculty training videos were created to further help with the implementation of the SynBio CURE.