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Are your ChIP antibodies skewing your data?
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This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"It’s a feared moment for every scientist: the discovery that years of painstaking research has led to results that can't be repeated. Many think that poorly characterized antibodies have contributed to this reproducibility crisis more than any other laboratory tool. A new study published in Molecular Cell supports this hypothesis, at least in the context of chromatin immunoprecipitation. Although accurate ChIP interpretation depends on near-perfect antibody specificity, the report shows that many of these reagents are far less capable than their advertising suggests, which calls into question several widely accepted paradigms on genomic regulation. The study focused on histone post-translational modifications; specifically all three methylation states of lysine 4 on histone H3. Through ChIP experiments, H3K4 methylation has been strongly linked to transcriptional control..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

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