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Overview:
In this module, students design and implement a strategy to identify yeast deletion strains by colony PCR. At the end of this module, students should be able to:design oligonucleotide primers to amplify specific DNA sequences with PCRexplain how changes to the annealing and extension times affect the production of PCR productsuse PCR to distinguish mutant yeast strains with different genotypesThis module is part of a semester-long introductory lab course, Investigations in Molecular Cell BIology, at Boston College.
Subject:
Biology, Genetics
Level:
Community College / Lower Division, College / Upper Division, Career / Technical
Material Type:
Module
Author:
Date Added:
08/29/2018
License:
Creative Commons Attribution Non-Commercial Share Alike Creative Commons Attribution Non-Commercial Share Alike
Language:
English
Media Format:
Downloadable docs, Video

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