Biology is designed for multi-semester biology courses for science majors. It is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand. To meet the needs of today’s instructors and students, some content has been strategically condensed while maintaining the overall scope and coverage of traditional texts for this course. Instructors can customize the book, adapting it to the approach that works best in their classroom. Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand—and apply—key concepts.

By the end of this section, you will be able to:Describe gel electrophoresisExplain molecular and reproductive cloningDescribe uses of biotechnology in medicine and agriculture

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"The spread of antibiotic resistance is one of the most pressing threats facing global health. Every year, approximately 700,000 deaths worldwide can be traced to antibiotic resistance. That makes it crucial to identify antibiotic resistance genes (ARGs) and their transmission between humans and the environment. Unfortunately, because they rely on curated databases and are not sensitive to certain mutations, many methods can overlook novel ARGs. Now, a new machine learning method called HMD-ARG could provide researchers with a more powerful alternative. Taking sequence encoding as input, it determines whether an input sequence is an ARG, what antibiotic family the ARG is resistant to, its mechanism of resistance, and whether it is intrinsic or acquired, and even the sub-class of antibiotic the ARG resists, if it happens to be a beta-lactamase, all without querying against existing sequence databases. The HMD-ARG database is the largest of its kind..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

In the video you can find some hints to design a primers for clone your GOI in a plasmid using restriction enzyme and ligase cloning approach

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Formalin-fixed, paraffin-embedded (FFPE) tissue is the gold standard for pathology tissue storage, making FFPE tissue libraries rich repositories for identifying and analyzing the bacterial microbiomes that stretch across the human body. Unfortunately, various facets of the FFPE process can compromise the integrity of tissue for this type of analysis. including DNA damage, susceptibility to contamination, and the lack of suitable DNA extraction methods. A new study proposes a system called Protoblock for standardizing and optimizing FFPE tissue-based research. A Protoblock is generated by embedding a known number of fixed cells in a molded agar matrix. After the agar solidifies, the block is processed following routine FFPE protocols and verified by microscopy. Experiments confirmed the quality and condition of DNA purified from Protoblocks, revealing important calibration information, such as how DNA damage evolves over fixation time. and how host DNA and sample prep method might bias bacterial analysis..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"The recent outbreak of SARS-CoV-2 infections has strained healthcare systems worldwide and diagnostic testing is a critical part of any pandemic management plan. PCR tests on clinical samples collected by health care professionals (HCPs) is currently the gold standard, but patient self-sampling may facilitate increased testing without adding strain or risk of exposure for HCPs. A recent study tested the sensitivity, feasibility, and acceptance of self-collected oropharyngeal samples. Hospitalized SARS-CoV-2-infected patients collected two self-administered samples and filled out a questionnaire. Researchers also collected HCP-administered samples for comparison. While the HCP-collected samples had the highest estimated sensitivity compared to each of the self-collected samples, at 88%, 78%, and 77% respectively, using both self-collected sample results together increased their estimated sensitivity to 88%, which is comparable to the HCP-collected samples..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

In this module, students design and implement a strategy to identify yeast deletion strains by colony PCR. At the end of this module, students should be able to:design oligonucleotide primers to amplify specific DNA sequences with PCRexplain how changes to the annealing and extension times affect the production of PCR productsuse PCR to distinguish mutant yeast strains with different genotypesThis module is part of a semester-long introductory lab course, Investigations in Molecular Cell BIology, at Boston College.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Our genome is a lot like a book. When cells need information critical to their function, they must physically crack the genome open to arrive at the right chapter, or gene sequence. Often, the relevance of details from chapter one isn’t clear until chapter ten. Similarly, non-coding sequences often control the expression of genes far away in linear genomic distance but relatively close in three-dimensional space. Now, a new method of probing these regions could help scientists gain more information from much less starting material—and, in the process, help us learn more about the book of life. The technique is called HiCAR, short for Hi-C on Accessible Regulatory DNA. HiCAR builds off the Hi-C method, which uses high-throughput sequencing to detect how different regions of genomic DNA interact with each other. Specifically, HiCAR targets the regions of chromatin that are open and accessible to proteins with information about gene regulation..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.