This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Built environment microbiomes are shaped by their occupants and the outside environment. These microbiomes can have a profound impact on the health of its occupants. The International Space Station (ISS) is a uniquely sealed environment, with only the arrival of crewmembers and supplies introducing new microbes. Monitoring the ISS microbiome is important to ensure astronaut health and spacecraft integrity. So, a recent study used samples from two long-term projects, Microbial Tracking 1 and 2, which sampled the same surfaces over two 14-month-long periods. The ISS surface microbiome was dominated by microbes associated with human skin. The most represented groups were Staphylococcus and Malassezia among bacteria and fungi, respectively. Community abundances shifted over time, but did not differ between surfaces. Overall, the metabolism genes tended towards amino acid utilization rather than carbohydrate metabolism..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

Students investigate decomposers and the role of decomposers in maintaining the flow of nutrients in an environment. Students also learn how engineers use decomposers to help clean up wastes in a process known as bioremediation. This lesson concludes a series of six lessons in which students use their growing understanding of various environments and the engineering design process, to design and create their own model biodome ecosystems.

This includes materials to be used for a General Biology II course (or Introduction to Biology II course) for non-science majors.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"The ribosomal RNA (rRNA) gene approach to sequencing genetic material has revolutionized microbiome science. But it isn’t perfect. The method relies on the assumption that counts of rRNA genes translate into microbial abundance. Exceptions to that rule, however, are known, such as the observation that rRNA gene counts can be higher in fast-growing microbes. Now, researchers report a new relationship between rRNA genes and cell volume that could help correct for biases inherent to microbiome studies. An analysis of previously reported data showed that the number of 16S or 18S RNA genes per cell follows an allometric power law of cell volume. Applying this relationship to a dataset for bacteria found in intertidal rocks allowed for more accurate biovolume and cell count distributions to be estimated for all taxa detected. The development of more comprehensive cell-size databases could help strengthen the bias-correcting relationship and boost the power of current microbiome analyses..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

Students design and conduct experiments to determine what environmental factors favor decomposition by soil microbes. They use chunks of carrots for the materials to be decomposed, and their experiments are carried out in plastic bags filled with dirt. Every few days students remove the carrots from the dirt and weigh them. Depending on the experimental conditions, after a few weeks most of the carrots will have decomposed completely.

This video segment adapted from A Science Odyssey tells the story of researcher Sir Alexander Fleming, whose luck and scientific reasoning led to the groundbreaking discovery of penicillin.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"All living things need phosphorus to survive. However, its low availability in soil is often a limiting factor for plant and microbial growth. Microorganisms in the plant root-soil interface (rhizosphere) can convert non-labile phosphorus into bioavailable forms. One way microbes do this is the mineralization of organic phosphorus compounds like phytate. Rising atmospheric CO₂ levels may accelerate mineralization, but the molecular mechanisms are not yet understood. Recent research confirmed that elevated CO₂ (eCO₂) increased the mineralization of phytate in the rhizosphere of wheat. Tracing the carbon flow showed that plants grown under eCO₂ increased the release of bioavailable carbon belowground, which corresponded to increased microbial growth and altered community composition. The bacterial community under eCO₂ favored groups of bacteria capable of degrading aromatic phosphorus compounds and the mycorrhizal fungi benefited from the increased supply of phosphorus and carbon..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

A Curriculum for Enhancing Farmer Microbial Literacy

Short Description:
The primary purpose of this course is to render the invisible world of microbes visible to farmers. This course provides scientific-based instruction exploring how microbes can assist agriculture. Although the course is designed for farmers, this material can also help extension agents, curious citizen scientists, and other non-experts interested in enhancing their microbial literacy.

Long Description:
The invisibility, complexity, and general opaqueness of soil microbial life makes learning about the “black box of soil” difficult. To address this challenge, my project-based biology senior thesis endeavors to develop a biology-based curriculum for farmers to increase microbial literacy. A transition to a microbially centric agriculture necessitates the development of a new knowledge infrastructure. My senior thesis project aspires to contribute to this new, necessary, and expanding infrastructure. It is critical that farmers recognize their unique role as stewards of not only plants and animals but also as shepherds of an unfathomably complex, necessary, and wondrous herd of microbes.

Word Count: 10617

(Note: This resource's metadata has been created automatically by reformatting and/or combining the information that the author initially provided as part of a bulk import process.)

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Natural microbial systems are all around us, from nutrient-rich soil to churning seawater to our own bodies. These complex systems include bacteria, archaea, viruses, and microbial eukaryotes. One of the best methods available for analyzing these systems is shotgun sequencing, which generates vast quantities of DNA sequence data. However, current data annotation methods don't include a dedicated way to find eukaryotic sequences. Now, researchers have introduced a bioinformatics method called EukDetect. EukDetect uses a database of over 500,000 universal marker genes from 241 conserved gene families across thousands of eukaryotes. Broad taxonomic coverage and accurate identification of low-abundance and high-similarity sequences were possible with EukDetect, and bacterial contamination was no obstacle to identifying eukaryotic species. EukDetect highlights information that could be missed or obscured in standard shotgun sequence analysis..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Plants are shaped by the many microbes they host. But scientists are only beginning to understand how, especially in underexplored plant structures like aerial roots. A new study shows that the mucilage secreted by these roots can create a microbiome unlike that found in underground roots and nurture an environment that caters to beneficial, nitrogen-fixing bacteria. Researchers made these discoveries by examining the aerial roots of pink lady shrubs—a fast-growing invasive plant. Metabolite profiling of aerial root mucilage revealed a rich cocktail of nutrients that would be expected to support an equally rich variety of microbes. But genomic analyses suggested a mucilage community dominated by nitrogen-fixing diazotrophs. This homogeneous community structure was linked to the presence of the fungus C. raphigera. The antibacterial activity of this fungus was such that only diazotrophs were allowed to thrive, to the benefit of the pink lady shrubs..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

Weird and wild fungi are everywhere, especially when it’s wet out. Given the chance to explore and observe fungi, students will notice them everywhere. Exploring fungi will also lead students to appreciate how fungi function in ecosystems as decomposers and other important roles. Recent discoveries in science have found that huge underground networks of thin, branching tubes of mycelium, the white tubes that are the main growing part of a fungus, provide key links between plants and the rest of the ecosystem.

In this Focused Exploration activity, students begin by observing fungi. Then, they learn that mushrooms are the fruiting bodies of fungi. Students use a simple key to identify types of fungi, record data, then regroup, and discuss patterns of where fungi grow. Next, they learn about how fungi digest what they live on, discuss fungal impacts on ecosystems, and reflect on fungi roles in decomposition.

In this 6th grade science class, students observe mushrooms growing wild in the garden, handle examples of common edible mushrooms and learn about the lifecycle of fungi.

Students conduct experiments to determine what environmental factors favor decomposition by soil microbes. They use chunks of carrots for the materials to be decomposed, and their experiments are carried out in plastic bags filled with dirt. Every few days students remove the carrots from the dirt and weigh them. Depending on the experimental conditions, after a few weeks most of the carrots have decomposed completely.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Plants are intimately connected to the microbiomes they host. These microbial communities provide plants with nutrients. and protection from environmental stress. While scientists know that plant-derived factors shape the composition of microbiomes. it’s unclear whether host evolution also plays a role. To find out, researchers recently investigated seeds of the genus Oryza, the common rice plant. They examined the effects of speciation and domestication of rice on seed microbiomes. and found that speciation gave rise to distinct communities of bacteria and fungi in seeds. Similarly, domestication tended to produce variations in the composition of fungal communities while conserving bacterial communities. These findings indicate that while evolutionary processes can affect microbiome composition in random fashion. the environmental changes that accompany domestication contribute to the assembly of microbiomes in deterministic ways..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This lesson plan explains how seeds can be dispersed, describes seed adaptations to ensure dispersal and explain the importance of this process.

The purpose of this lesson plan is to learn about the process and importance of decomposition while creating actual compost.

Looking at lichen through a hand lens can be like looking at life-forms from an alien planet. In this activity, students focus closely on lichen and get turned on to its different strange and interesting forms. One reason for spending time learning about lichens is that they can be found just about anywhere, so students can keep investigating lichen after they leave your program. Students observe and explore this “weird organism” that grows on rocks and trees and wonder what it is. They learn that it’s a lichen, use a key to identify three types of lichen, reflect on the symbiotic relationship of fungi and algae that make up lichens, and finally search for evidence of lichen succession. After this activity, students will likely begin to notice lichens everywhere, and will be motivated to continue their explorations.

In this lesson, students will be amateur mycologists--collecting and analyzing various mushrooms. Through observation and discussion, students will gain knowledge of the basic anatomy of mushrooms, their life cycle, and their method of reproduction through spores. Students will learn to create spore prints of mushrooms and label and preserve their spore prints, just like a mycologist. Students also will learn that by comparing spore prints, they can identify different mushroom species.

This webpage gives detailed information on the following.
1 Etymology
2 Identification
3 Classification
4 Morphology
5 Growth
6 Nutrition
7 Edible mushrooms
8. Medicinal properties & other uses

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Nanotechnology has enabled numerous advances in communications, medicine, energy, and agriculture. But as the proliferation of nanomaterials ramps up, unintended consequences are becoming increasingly visible. A recent study examined the effects of silver nanoparticles on soils supporting the growth of maize. Findings revealed a 30% decrease in the relative abundance of members of the archaea community, which are important for nitrogen cycling in soil, vital for plant growth. Nanosilver also increased the abundance of certain possibly phytopathogenic fungi, possibly by eliminating bacteria that normally keep these harmful fungi in check. Finally, increased root growth seems unsustainable because it is probably caused by these stress factors, and not by beneficial effects. While this study was limited to relatively small, pot experiments, the findings suggest that the microbiome is an essential inclusion for studying the long-term effects of nanomaterials on the environment..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.