TABLE OF CONTENTS
Introduction
Schedule
Best Laboratory Practices
Working in a Microbiology Laboratory

Exercises
Preparation of a Culture Medium
Culturing and Handling Microorganisms
Isolation of a Pure Culture
Counting Bacterial Populations
Controlling Microorganisms
Disinfectants
Antimicrobial Agents: Susceptibility Testing
Hand Washing
The Lethal Effects of Ultraviolet Light
Selection of Fungi from Air
Microscopy
Morphology and Staining of Bacteria
Microbial Metabolism
Enzyme Assay
Metabolic Capabilities
Microbial Genetics
Transformation of Escherichia coli
Isolation of Plasmid DNA
Restriction Enzyme Digestion of Plasmid DNA and Gel Electrophoresis

Appendices
A – List of Organisms Used Throughout the Semester
B – Vocabulary
C – Media
D – Intro to Micropipettes
E – Microscopy Pocket Guide
F – References and Supplemental Materials

This textbook is designed for instructional laboratories of undergraduate general microbiology courses. Basic microbiological laboratory approaches are emphasized, as well as the diversity of microbes. Morphologies, physiologies, methods of microbial isolation, identification, and characterization are explored. The manual includes an unknown identification project with instructions for writing a scientific report detailing the project and its results.

Hi. This OER contains PowerPoint Presentation slides for the Microbiology OpenStax textbook. Chapters 1 - 10 have been uploaded to this platform. All PowerPoint presentations will be refined during implementation. In the meantime, please feel free to use these resources and modify to your liking as I have a different arrangement of slides that are most suitable to my student's needs and course learning outcomes. Thank you for taking time to stop by!  "OpenStax Microbiology Slides" by Adronisha Frazier, Louisiana Community and Technical College System, Northshore Technical Community College is licensed under CC BY-SA 4.0.

This poster set was designed for display in a microbiology teaching laboratory. The consistent 12 step layout contributes to a cohesive appearance. The color hints on the bottom of each poster shows what students should expect as results from staining. These posters were designed to be printed as 18x24 in, or displayed digitally.

Microorganisms are the most abundant form of life on Earth and in recent decades it has become increasingly clear that their collective activities are one of the dominant forces shaping the planet.

This book provides earth scientists with an introduction to microbiology and a look at the ways microorganisms are important to their area of expertise. The first part of this book summarizes some basic information about microorganisms, including a discussion of their diversity, physical properties, and metabolisms. From there, the second and third portions of the book are organized around the two-way interactions between microorganisms and their environments. The second portion of the book considers the ways that environmental conditions help determine distributions of microbial activity, including chapters focused on thermodynamic, kinetic, and biological factors. The third and final portion of the book examines the impacts of microbes on their environments. These impacts are placed within the context of earth system science, with chapters focused on impacts to the lithosphere, atmosphere, and hydrosphere. In these chapters, emphasis is placed on microbial impacts to greenhouse gas levels and the quality of water resources, underscoring the relevance of microbiology to environmental concerns of keen interest in the earth science community and beyond.

This book is specifically designed for earth science students and can provide a helpful free resource for students in Geomicrobiology courses. However, portions of the book can also have value for students and professionals from any field who are interested in environmental microbiology.

This group learning activity involves students in an engaging review of concepts of microbiology. The review is set up as a power point presentation in the style of the "Jeopardy" gameshow where students groups compete against each other to answer review questions.

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This group learning activity involves students in an engaging review of concepts of microbiology. The review is set up as a power point presentation in the style of the "Jeopardy" gameshow where students groups compete against each other to answer review questions.

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Bacillus cereus cells were grown in broth culture for 48 hours at 30 degrees Celsius. The cells were heat-fixed to a slide and stained with malachite green (endospores) and safranin red (vegetative cells) prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Bacillus megaterium cells were grown in broth culture for 5 days at 30 degrees Celsius. The cells were heat-fixed to a slide and stained with malachite green (endospores) and safranin red (vegetative cells) prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Bacillus subtilis cells were grown in broth culture for 48 hours at 30 degrees Celsius. The cells were heat-fixed to a slide and stained with malachite green (endospores) and safranin red (vegetative cells) prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Bacillus subtilis cells were grown in broth culture for 5 days at 30 degrees Celsius. The cells were heat-fixed to a slide and stained with malachite green (endospores) and safranin red (vegetative cells) prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Bacillus subtilis cells grown in broth culture overnight at 30 degrees Celsius. The cells were heat-fixed to a slide Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 100X total magnifcation on a brightfield microscope. The subject is Bacillus subtilis cells grown in broth culture overnight at 30 degrees Celsius. The cells were heat-fixed to a slide Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Candida albicans cells grown in broth culture at 30 degrees Celsius. The cells were heat-fixed to a slide and Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Candida albicans cells grown in broth culture at 30 degrees Celsius. The cells were heat-fixed to a slide and Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is Candida albicans cells grown on YPD agar at 30 degrees Celsius. The cells were heat-fixed to a slide and Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 100X total magnifcation on a brightfield microscope. The subject is Candida albicans cells grown in broth culture at 30 degrees Celsius. The cells were heat-fixed to a slide and Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 400X total magnifcation on a brightfield microscope. The subject is Candida albicans cells grown in broth culture at 30 degrees Celsius. The cells were heat-fixed to a slide and Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 400X total magnifcation on a brightfield microscope. The subject is Candida albicans cells grown in broth culture at 30 degrees Celsius. The cells were heat-fixed to a slide and Gram stained prior to visualization.Image credit: Emily Fox

This micrograph was taken at 1000X total magnifcation on a brightfield microscope. The subject is unidentified coccus cells from a contaminant colony grown on nutrient agar at 30 degrees Celsius. The cells were heat-fixed to a slide and Gram stained prior to visualization.Image credit: Emily Fox