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Biochemistry Laboratory
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The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.
This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format.
Acknowledgments
Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan.

Subject:
Biology
Life Science
Material Type:
Full Course
Provider Set:
MIT OpenCourseWare
Author:
Taylor, Elizabeth
Date Added:
02/01/2009
Biological Processes: Putting Microbes to Work
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Educational Use
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Students learn the fundamentals of using microbes to treat wastewater. They discover how wastewater is generated and its primary constituents. Microbial metabolism, enzymes and bioreactors are explored to fully understand the primary processes occurring within organisms.

Subject:
Applied Science
Engineering
Environmental Science
Material Type:
Lesson Plan
Provider:
TeachEngineering
Provider Set:
TeachEngineering
Author:
Dayna Lee Martinez
Tapas K. Das
Date Added:
09/18/2014
Biology
Unrestricted Use
CC BY
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Biology is designed for multi-semester biology courses for science majors. It is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand. To meet the needs of today’s instructors and students, some content has been strategically condensed while maintaining the overall scope and coverage of traditional texts for this course. Instructors can customize the book, adapting it to the approach that works best in their classroom. Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand—and apply—key concepts.

Subject:
Biology
Life Science
Material Type:
Full Course
Provider:
Rice University
Provider Set:
OpenStax College
Date Added:
08/22/2012
Biology, The Cell, Metabolism, Enzymes
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CC BY-NC-SA
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By the end of this section, you will be able to:Describe the role of enzymes in metabolic pathwaysExplain how enzymes function as molecular catalystsDiscuss enzyme regulation by various factors

Subject:
Applied Science
Biology
Life Science
Material Type:
Module
Author:
Tina B. Jones
Date Added:
08/15/2019
Biology, The Chemistry of Life, Biological Macromolecules, Proteins
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CC BY-NC
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By the end of this section, you will be able to:Describe the functions proteins perform in the cell and in tissuesDiscuss the relationship between amino acids and proteinsExplain the four levels of protein organizationDescribe the ways in which protein shape and function are linked

Subject:
Applied Science
Biology
Life Science
Material Type:
Module
Date Added:
07/10/2017
Biomolecular Kinetics and Cellular Dynamics (BE.420J)
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CC BY-NC-SA
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This subject deals primarily with kinetic and equilibrium mathematical models of biomolecular interactions, as well as the application of these quantitative analyses to biological problems across a wide range of levels of organization, from individual molecular interactions to populations of cells.

Subject:
Applied Science
Biology
Engineering
Life Science
Material Type:
Full Course
Provider Set:
MIT OpenCourseWare
Author:
Tidor, Bruce
Wittrup, Karl
Date Added:
09/01/2004
Cellular Respiration
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Cellular respiration is the process by which our bodies convert glucose from food into energy in the form of ATP (adenosine triphosphate). Start by exploring the ATP molecule in 3D, then use molecular models to take a step-by-step tour of the chemical reactants and products in the complex biological processes of glycolysis, the Krebs cycle, the Electron Transport Chain, and ATP synthesis. Follow atoms as they rearrange and become parts of other molecules and witness the production of high-energy ATP molecules.

Subject:
Life Science
Material Type:
Lecture Notes
Simulation
Provider:
Concord Consortium
Provider Set:
Concord Consortium Collection
Author:
The Concord Consortium
Date Added:
01/13/2012
DNA Forensics and Color Pigments
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Educational Use
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Students perform DNA forensics using food coloring to enhance their understanding of DNA fingerprinting, restriction enzymes, genotyping and DNA gel electrophoresis. They place small drops of different food coloring ("water-based paint") on strips of filter paper and then place one paper strip end in water. As water travels along the paper strips, students observe the pigments that compose the paint decompose into their color components. This is an example of the chromatography concept applied to DNA forensics, with the pigments in the paint that define the color being analogous to DNA fragments of different lengths.

Subject:
Applied Science
Engineering
Genetics
History
History, Law, Politics
Life Science
Physical Science
Physics
Material Type:
Activity/Lab
Provider:
TeachEngineering
Provider Set:
TeachEngineering
Author:
Mircea Ionescu
Myla Van Duyn
Date Added:
09/18/2014
Enzymatic Proteins - How They Regulate Life
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CC BY-NC
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In this seminar students will explore the hacking of glue! You will inquire about the way in which enzymes are a part of chemical reactions in the biological sense through simulations.  Experimental investigations will lead to the understanding of the denaturing process of enzymes.StandardsBIO.A.2.2.2 Describe how biological macromolecules form from monomers.BIO.A.2.2.3 Compare and contrast the structure and function of carbohydrates, lipids, proteins, and nucleic acids in organisms.

Subject:
Biology
Life Science
Material Type:
Lesson Plan
Author:
Bonnie Waltz
Deanna Mayers
Tracy Rains
Date Added:
10/08/2017
Forest floor microbes produce tough biofilm breaker
Unrestricted Use
CC BY
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This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Biofilms are the slimy cities some microbes form when they invade a surface. Conventional cleaning products are generally good at breaking up biofilms. But they tend to be harsh on the environment. And while natural products are a good alternative, it takes multiple enzymes to break up the strong polymers that make bacteria stick. But researchers are confident that a natural solution does exist. One team searched the forest floor in the Netherlands for microbes that might produce an all-in-one biofilm-busting enzyme. To coax those microbes out, they enriched forest litter with an especially tough biopolymer produced by forest bacteria: Acidobacteria. Microbes that could thrive in that environment likely produced enzymes strong enough to degrade the biopolymer blend. Analyses indicated the predominance of four bacterial phyla. More importantly, they revealed the main type of enzyme these bacteria secreted: glycoside hydrolases..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

Subject:
Biology
Life Science
Material Type:
Diagram/Illustration
Reading
Provider:
Research Square
Provider Set:
Video Bytes
Date Added:
10/28/2020
Kitchen Chemistry
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CC BY-NC-SA
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This seminar is designed to be an experimental and hands-on approach to applied chemistry (as seen in cooking). Cooking may be the oldest and most widespread application of chemistry and recipes may be the oldest practical result of chemical research. We shall do some cooking experiments to illustrate some chemical principles, including extraction, denaturation, and phase changes.

Subject:
Chemistry
Physical Science
Material Type:
Full Course
Provider Set:
MIT OpenCourseWare
Author:
Christie, Patricia
Date Added:
02/01/2009
Lessons 1: Qualitative Kinetics: Examining the effect of an enzyme on a reaction
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Educational Use
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Chemical kinetics and buffers are two topics that are extremely difficult for students to understand. Combining the two topics will allow for a staggered, repetitive approach to teaching students to understand of how these two topics in chemistry actually work. Students will both qualitatively and quantitatively track the effect and enzyme has on a reaction, calculate the reaction rate and buffer capacity. Students will use a variety of lab techniques including calculations using Beer’s Law and spectrophotometry.

Subject:
Chemistry
Physical Science
Material Type:
Lesson Plan
Provider:
North Carolina State University
Provider Set:
Kenan Fellows Program for Curriculum and Leadership Development
Author:
Chris England
Date Added:
03/03/2016
Let's Learn about Chirality
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CC BY-NC-SA
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Let's Learn about Chirality is a video-based module that introduces the concept of chirality and stereoisomers to students. The module is an Open Educational Resources #OER containing short videos and fun quizzes* that you can take to test your understanding. In this module, you will learn to use different terms for stereoisomers and chiral molecules, recognize characteristics of stereoisomers, construct a model of 2-chlorobutane, identify the chiral carbons in chemical structures and assign absolute configurations to a molecule. The concluding video will briefly explain how chirality affects the chemistry of substances & pharmaceuticals. Click on View Resources and let's learn about Chirality.*For teachers, the Genial.ly quizzes are re-useable for your classes.*

Subject:
Biology
Chemistry
Health, Medicine and Nursing
Life Science
Material Type:
Assessment
Interactive
Lecture
Module
Author:
Aisyah Saad Abdul Rahim
Chee Yan Choo
Date Added:
06/07/2021
Molecular explorers: Adventures in green science
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CC BY-NC
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Join our team of young explorers on their mission to save nature from pollution! When this group of friends discovers the impact people have on their environment, they embark on a quest for justice and answers that quickly throws them into a whirlwind of adventures. Their goal: to find powerful molecules capable of solving the pollution problem. Get ready for an exciting journey through unexplored territories and discover the microscopic power hidden all around us. Be part of our quest for a greener world!

Subject:
Applied Science
Environmental Science
Material Type:
Diagram/Illustration
Game
Interactive
Author:
RadicalZ Project
Date Added:
09/19/2024